Differences in Protease Expression of Mutants Bacillus licheniformis F11
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چکیده
منابع مشابه
Production of Extracellular Protease and Determination of Optimal Condition by Bacillus Licheniformis BBRC 100053 (RESEARCH NOTE)
The production of protease by Bacillus licheniformis BBRC 100053 was studied. The most appropriate medium for the growth and protease production is composed of: lactose 1%, yeast extract 0.5%, peptone 0.5%, KH2PO4 0.1%, MgSO4.7H2O 0.02%. Enzyme production corresponded with growth and reached a maximums level (589 U/ml) during the stationary phase at 35°C, pH equivalent to 10 and with 150 rpm af...
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In this work the subC gene from Bacillus licheniformis encoding subtilisin was cloned into the nisin-controlled expression (NICE) vectors (pNZ8048 and pNZ8148) with or without the signal peptide SP Usp45 directing extracellular secretion via Sec machinery. Extracellular protease production and activity was tested using Lactococcus lactis NZ9000 as host, which could be used for rennet production...
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in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...
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Alkaline Protease enzyme produced from Bacillus Licheniformis PTCC1331 in optimum Condition(Temperature 37 ?C, 150 rpm, PH=10), is immobilized in different concentrations of Sodium Alginate using entrapment method, to increase its thermal resistance in detergents, and its activity is measured thereafter. Maximum enzymatic activity of 46 was obtained in 2%w/v Sodium alginate solution. Investiga...
متن کاملOptimization of protease secretion in Bacillus subtilis and Bacillus 1 licheniformis by screening of homologous and heterologous signal peptides
3 Henkel AG & Co. KGaA, Biotechnology, D-40191 Düsseldorf, Germany 11 12 13 14 * Corresponding author: 15 Karl-Erich Jaeger 16 Phone: +49 2461 613716 17 Fax: +49 2461 612490 18 E-mail: [email protected] 19 20 21 22 23
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ژورنال
عنوان ژورنال: Biosciences, Biotechnology Research Asia
سال: 2015
ISSN: 0973-1245
DOI: 10.13005/bbra/1871